Table 1.
Sequences of qRT-PCR primers used in this study
| Gene | GenBank no. | Primer sequence (5′-3′) | Product length |
|---|---|---|---|
| CPEB1 | NM_001079533 | Forward: GTCCTCCCAAAGGTAATATGCC | 262 |
| Reverse: TGCAGAGCACCGACAAACA | |||
| SIRT1 | NM_001142498 | Forward: CCCCATGAAGTGCCTCAGAT | 223 |
| Reverse: TGGGTGGCAACTCTGACAAA | |||
| CD133 | NM_001145847 | Forward: TCACCAGCAACGAGTCCTTC | 270 |
| Reverse: GGTTTGCACGATGCCACTTT | |||
| CD24 | NM_001291737 | Forward: GCTCCTACCCACGCAGATTT | 162 |
| Reverse: GAGACCACGAAGAGACTGGC | |||
| EpCAM | NM_002354 | Forward: CCATGTGCTGGTGTGTGAAC | 159 |
| Reverse: GAAGTGCAGTCCGCAAACTT | |||
| OCT4 | NM_001173531 | Forward: ATGTGGTCCGAGTGTGGTTC | 232 |
| Reverse: GAGACAGGGGGAAAGGCTTC | |||
| NANOG | NM_001297698 | Forward: AGACAAGGTCCCGGTCAAGA | 246 |
| Reverse: AGGCATCCCTGCGTCACAC | |||
| SOX2 | NM_003106 | Forward: TTTGTCGGAGACGGAGAAGC | 237 |
| Reverse: TAACTGTCCATGCGCTGGTT | |||
| LIN28 | NM_024674 | Forward: ACCGGACCTGGTGGAGTATT | 199 |
| Reverse: GCGGACATGAGGCTACCATA | |||
| GAPDH | NM_001256799 | Forward: GAGAAGGCTGGGGCTCATTT | 231 |
| Reverse: AGTGATGGCATGGACTGTGG |