Fig. 6. Epigenomic alterations contributing to liver fibrosis are reverted in anti-miR-873-5p-treated-mice through the restoration of GNMT, SAMe metabolism, and transmethylation flux.

(a) DNA CpG (5mC) methylation in Ctrl and BDL/anti-miR-873-BDL livers (N = 4). qPCR and WB analysis of genes implicated in DNA methylation (b). qPCR analysis of indicated genes susceptible of promoter hypermethylation related to cholangiocyte proliferation and bile acid metabolism (c-e) in miR-Ctrl and anti-miR-873-5p BDL/Mdr2^-/- mice (N = 4 (BDL) and N = 5 (Mdr2^-/-). (f) WB analysis of EZH2 in anti-miR-873-5p-BDL/Mdr2^-/- mice (N = 4 (BDL) and N = 5 (Mdr2^-/-). Densitometry analyses of WB are shown in Supp. Fig. 7C. Data normalized as fold change vs. control. Error bars represent the means ± SEM. Statistical significance was determined by the Student’s t-test or ANOVA when more than two groups were compared. p < 0.05*; p < 0.01**; p < 0.001***