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. 2017 Jun 29;313(4):L699–L709. doi: 10.1152/ajplung.00197.2017

Fig. 2.

Fig. 2.

Toll-like receptor-2 (TLR2) and -9 expression and nucleotide-binding domain and leucine-rich repeat-containing gene family, pyrin domain containing 3 (NLRP3) inflammasome activation in pneumonia. A: Western analysis of TLR2 and TLR9 protein levels in total lung homogenate in mice. TLR protein expression showed a robust increase at day 1 then returned to baseline at day 3 postinfection. B: Western analysis of NLRP3 and IL-1β proteins. Tubulin is loading control. C: receptor for advanced glycation end points (RAGE) protein in BAL fluid determined by Western analysis. Coomassie staining is loading control. Release of RAGE indicates alveolar injury. D: mean immunoblot densities for TLR2 compared with corresponding tubulin. E: TLR9 histogram relative to tubulin. F: relative densities of IL-1β relative to tubulin. G: IL-18 protein levels. H: NLRP3 protein levels relative to tubulin. I: RAGE protein levels in BAL fluid. Each bar in D–I represents means ± SE for each group; n = 6; *P < 0.05 by one-way ANOVA.