Fig. 3.

Lung cellular antioxidant defenses in S. aureus pneumonia. Antioxidant enzyme mRNA levels for were measured in total purified RNA by quantitative RT-PCR and expressed as means ± SE. A: lung heme oxygenase-1 (HO-1) mRNA levels in inoculated mice peaked at day 1. B: SOD2 mRNA transcript expression increased by day 1. C: peroxyredoxin 3 (Prdx3) mRNA transcript, the mitochondrial isoform, increases significantly at day 2. D: glutathione peroxidase 4 (Gpx4) mRNA transcript levels, the mitochondrial isoform, increases significantly at days 1 and 2 of pneumonia. *P < 0.05 vs. time 0 by one-way ANOVA; n = 6 mice per group.