Fig. 6.

Mitochondrial network protein expression. A: lung dynamin-1-like protein (Drp) and mitochondrial fission factor (Mff) protein levels by Western analysis. These mitochondrial fission proteins compared with outer membrane protein VDAC respond at days 1 and 2 postinoculation. B: scatterplots for Drp1 showing postinoculation effect. C: scatterplots for Mff showing an increase at day 1. D: mitofusin-1 and -2 (Mfn1 and Mfn2) expression by Western analysis relative to VDAC. E: scatterplots for Mfn1 showing stability after inoculation. F: scatterplots for Mfn2 showing a response at day 3 postinfection. Data expressed as protein density relative to VDAC. For scatter plots in B, C, E, and F, bars are means ± SE for n = 6 per time point; *P < 0.05 vs. time 0 by one-way ANOVA.