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. 2018 Sep 20;8:14090. doi: 10.1038/s41598-018-32497-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of AXT on infiltration of immune cells in chronic-binge ethanol induced alcoholic liver injury in mice. (A) Neutrophil levels in blood of pair-fed mice, ethanol-fed mice and ethanol-fed with AXT mice for 10 days and gavaged a single dose of ethanol (5 g/kg bodyweight) or isocaloric maltodextrin. (B) mRNA expression levels of Ly6g, a marker of neutrophil, in the pair-fed mice, ethanol-fed mice and ethanol-fed with AXT mice. (C) Immunohistochemical analysis of Ly6g confirmed in the pair-fed mice, ethanol-fed mice and ethanol-fed with AXT mice (scale bars, 100 μm). (D) The expression of VCAM-1 were determined in the total protein extracts of mice liver tissues by Western blotting. n = 8 per group; means ± SD of the mean. ^#p < 0.05, ^##p < 0.01 and ^###p < 0.001 pair-fed vs. ethanol-fed, *p < 0.05, **p < 0.01 and ***p < 0.001 ethanol-fed vs. ethanol-fed with AXT.