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. 2018 Sep 20;8:14116. doi: 10.1038/s41598-018-32349-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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B. divergens forms inside and outside erythrocytes were identified in in vitro cultures by Giemsa staining. (a) A free merozoite invading a human erythrocyte. Panels b–j show different stages within the human erythrocyte. (b) Single round trophozoite. (c) Paired pyriforms, a stage formed by two attached pear-shaped sister cells. (d) Double round trophozoites, (e) Double paired pyriforms. (f) Tetrad (g). Quadruple round trophozoites. (h) Multiple parasites. (i) Double unattached pyriforms. (j) Quadruple unattached pyriforms. (k) Intact paired pyriform outside the erythrocyte. (l) Intact tetrad outside the erythrocyte. Slides were examined with a Primo Star microscope (Zeiss, Germany) at 100X magnification.