Fig. 9.

VGLUT2-IR terminals were located in close apposition to tanycytes in the median eminence. A: vimentin-IR tanycyte processes (pseudo-colored blue) extend into the external zone of the median eminence in close proximity to CRH fibers (pseudo-colored red) and VGLUT2-IR (pseudo-colored green). Scale bar = 100 µm. B: higher-magnification image of dashed box in A. Arrows indicate VGLUT2 terminals surrounding tanycyte processes. An asterisk denotes close association of CRH fiber (pCRH-AAV-eGFP from PVN) with VGLUT2-IR. Scale bar = 25 µm.