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. 2018 Sep 20;9(10):949. doi: 10.1038/s41419-018-0995-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Knockout of PAR2 reduced YAP protein in vivo. a Protein and (b) mRNA expression of YAP in mouse colon was detected with western blotting or RT-PCR, respectively. c Immunohistochemical analysis of paraffin-embedded colon sections from wild-type and PAR2 knock-out mice. Scale bars represent 100 μm. d Number of colon epithelial cell with nuclear YAP was quantified. Data are shown as mean ± SEM. N.S., no significance; ***p < 0.001 vs. corresponding control