Fig. 5.

PAR2 activation induced dephosphorylation of YAP independent of Lats1. a HT-29 cells were pretreated with the inhibitor of protein synthesis, cycloheximide (CHX, 100 mg/ml). Then the cells were challenged with or without PAR2 inhibitor ENMD-1068 (1 mM) for different time periods. b HEK293 cells were serum-starved for 12 h and then stimulated with 100 μM PAR2-AP for different times. The levels of YAP, p909-Lats1, and Lats1 were measured with western blot. c Western blot was used to detect p909-Lats1 and Lats1 in HT-29–vector and HT-29–shPAR2 cells. d HEK293, A549, and HT-29 cells were pretreated with the inhibitor of protein synthesis, cycloheximide (CHX, 100 mg/ml), and then treated with OA (100 nM) for different time periods. Relative density of YAP from different cells was quantified (right). e A549 or HEK293 cells were pretreated with or without 100 nM OA and CHX, and then challenged with 100 µM PAR2-AP for different time periods. Relative density of YAP from different cells were quantified (right)