Table 1.
ITC affinity measurements of NRZ: BH3 motif interactions
| Peptide | WT NRZ KD (nM) |
NRZ R86A KD (nM) |
NRZ D79A KD (nM) |
|---|---|---|---|
| Bax | 688 ± 111 | 4600 ± 440 | 264 ± 42 |
| Bim | 41 ± 5 | 168 ± 8 | 13 ± 4 |
| Bad | 343 ± 48 | 4800 ± 88 | 3330 ± 41 |
| Puma | 36 ± 4 | 2770 ± 162 | 117 ± 5 |
| Bik | 12 ± 2 | 17 ± 3 | 14 ± 3 |
| Noxa | 142 ± 16 | 510 ± 41 | 335 ± 31 |
| Bcl-wav | 3570 ± 162 | NB | NB |
| Bid | 409 ± 55 | NB | 220 ± 16 |
| Bmf | NB | NB | NB |
| Beclin | NB | NB | NB |
26-mer peptides spanning the BH3-motyif of D. rerio pro-apoptotic Bcl-2 family members or Beclin-1 from zebrafish were employed. All KD values (in nM) are the means of three replicates with standard error
NB no binding