Fig. 2. RIF1 promotes NSCLC cell growth in vitro and in vivo.
a, b The silencing effect of the shRNA-RIF1 was confirmed by western blot in H1299 and SK-MES-1 cells. c Cell viability of H1299 and SK-MES-1 cells transfected with shRNA to RIF1 (RIF1 knockdown, RIF1 KD) or scrambled control vector (SCR), RIF1 overexpression construct (RIF1 over) or empty vector. d Colony formation assay of RIF1 knockdown or overexpressed H1299 and SK-MES-1 cells compared with scrambled controls and empty vector. e, f In all, 2 × 106 scrambled control and RIF1 stable knockdown H1299 cells were inoculated subcutaneously into the left and right flanks of nude mice. Tumor volume (e) and mass (f) of xenografts were evaluated. g Representative images of HE and IHC staining of the resected tumor. IHC analysis showed that RIF1 knockdown decreased the expression of the proliferation index Ki-67. Scale bar, 500 μm. Data were presented as means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001