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. 2018 Sep 20;9(10):942. doi: 10.1038/s41419-018-0972-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a, b Immunoprecipitation was performed with PP1 antibody and the precipitated protein complexes were analyzed by western blots with antibodies to RIF1 and AXIN in H1299 (a) and SK-MES-1 (b) cells. c, d RIF1-silenced H1299 (c) and SK-MES-1 (d) cells and the scrambled control cells were transfected with Flag-tagged AXIN plasmid. Immunoprecipitation was done with anti-FLAG M2 magnetic beads and the precipitated complexes, which were pull down by Flag-tagged AXIN were subjected to western blot with antibodies to RIF1, PP1 and p-Ser. e, f H1299 (e) and SK-MES-1 (f) cells were co-transfected Flag-tagged AXIN plasmid with control vector or RIF1 overexpression plasmid. Immunoprecipitation was performed with anti-FLAG M2 magnetic beads and the precipitated complexes were analyzed by western blots with antibodies to RIF1, PP1 and p-Ser (mouse IgG conjugated with magnetic beads being the negative control)