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. 2018 Sep 20;9(10):942. doi: 10.1038/s41419-018-0972-4

Fig. 7. RIF1 activates Wnt/β-catenin signaling through promoting PP1 to dephosphorylate AXIN.

Fig. 7

a Western blot analyses of protein expression of AXIN, β-catenin, p-β-catenin, MYC, cyclin D1 and β-actin in RIF1-silenced or overexpressed H1299 and SK-MES-1 cells with or without PP1 knockdown. b RT-qPCR analysis of the expression of AXIN in RIF1-overexpressed H1299 and SK-MES-1 cells. c Subcellular β-catenin localization was detected in the indicated cells by immunofluorescence staining. Scale bar, 10 μm. d, e The indicated cells were transfected with TOPflash or FOPflash and pRL-TK plasmids and then subjected to luciferase reporter assay 24 h post-transfection. The reporter activity was normalized to the activity of the pRL-TK reporter. f Colony formation assay of RIF1-overexpressed or control vector H1299 and SK-MES-1 cells with or without PP1 knockdown. g Representative images and quantification of spheres formed by RIF1-overexpressed or control vector H1299 and SK-MES-1 cells with or without PP1 knockdown. Scale bar, 100 μm. Data are expressed as means ± SD of three independent experiments. *P < 0.05, **P < 0.01. NS not statistically significant