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RNAase protection experiments performed with the 859-bp BamHI-SstI probe (A) or the 381-bp Exo-SstI probe (B). Total RNAs (20 μg) from various cell lines indicated above each panel were hybridized with the corresponding probe. In each case a protection experiment was also performed with tRNAs as a negative control. The sizes of the protected fragments are indicated. The three major start sites S1, S2, and S3 are also depicted.
