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RT-PCR detection of transcripts initiated at the P2 promoter. RNAs from COLO 320 (lane 1), HepG2 (lane 2), and NBE (lane 3) cells were retrotranscribed using the oligo dT primer and amplified using RT1 and RT2 primers. (M) Molecular size marker. Arrows point to the two fragments discussed in the text.
