FIG. 4.

(A) Complete sequence of the 925-amino acid FIR1 protein that is expressed from the YPT8-GLN3 intergenic region on chromosome V (GenBank accession number U18778; locus SCE9537; ORF YER032W). Underlined are amino acids 79 and 125, which are fused to the GAL4 activation domain in pGAD24-1 and pGAD24-4, respectively. (B) Map of the F1R1 locus as well as the gene disruption constructs, pFRlLEU2 and pFRITRPI, which were used for generating the Δfir1 and Δref2, Δfir1 strains used in this study (see Table 1 and Materials and Methods for details).