Fig. 3. BI 853520 treatment downregulates genes implicated in cell proliferation and upregulates genes implicated in immune response.

a, b List of biological processes enriched in downregulated and upregulated genes, respectively, upon treatment of mice bearing primary 4T1 tumors with 50 mg/kg BI 853520 dissolved in Natrosol-vehicle or vehicle alone for 5 days. All terms shown in the bar graphs are statistically significant (p value ≤0.05). The blue and red bars represent p values in −log 10 format and the fold enrichment for each term, respectively. The p values and enrichment were computed using Fisher’s exact test. c Box whisker plots showing the relative expression of genes involved in processes of cell cycle, positive regulation of mitotic cell cycle, and negative regulation of cell proliferation that were identified using gene set enrichment analysis. Relative gene expression was compared between vehicle-treated group (n = 3) and BI 853520-treated group (n = 6). The significance between the groups is shown as p values using an unpaired, two-tailed Student’s t test. ***p < 0.001, **p < 0.01, and *p < 0.05