FIG. 6.

Concomitant detection of mAb H5 and mAb 7C2 antigens of RNA polymerase II molecules with 10- and 5-nm gold particles, respectively, in cells transiently expressing the Us11 gene. Formaldehyde fixation, Lowicryl embedding, and uranyl acetate staining. Bars represent 0.5 μm. (a, b) Mixed 5-nm (arrows) and 10-nm gold particles are present over the clump of intermingled viral RNP fibrils (star) in (a) and the cluster of interchromatin granules (IG) in (b). Individual 5- and 10-nm gold particles are present in the surrounding nucleoplasm. CB: coiled body.