FIG. 3.

Transcriptional ability of Ala-substituted P mutants. (A) In vitro transcription reconstitution was carried out as described in Fig. 2. P60A, P62A, and P64A are single mutants where Ser60, Thr62, or Ser64 is mutated to Ala, respectively. The double mutants are P60A/64A (Ser60, Ser64 mutated to Ala), P60A/62A (Ser60, Thr62 mutated to Ala), and P62A/64A (Thr62, Ser64 mutated to Ala). P3A is the triple mutant where Ser60, Thr62, and Ser64 mutated to Ala. (B) Transcription of N-RNA using soluble PO and P3A. S stands for soluble.