Effects on the basal activity in the bladder strips with the PKC activator phorbol 12,13-dibutyrate (PDBu). Tone (left), and amplitude (middle), and frequency (right) of spontaneous contractions. PDBu showed a biphasic effect on the tone in control group, which was not observed in PBOO + P mice. A notable decrease in the frequency was observed in PBOO groups compared with those in control mice. Values are means ± SE. *P < 0.05, between control and PBOO + P groups; †P < 0.05, between control and PBOO + T groups.