FIG. 4.

Identification of an alternatively spliced ALDH3 transcript. The PCR products were amplified from 0.1 fig of total RNAs from various human tissues and cultured cells and analyzed by electrophoresis on a 2% agarose gel. The positive bands were sliced, subcloned, and sequenced. The sizes of the expected PCR products are indicated. Top and bottom bands are the regular and alternatively spliced transcript skipping exon 2, respectively. Minor 381-bp products derived from the transcripts using alternative splice acceptor site at the 5′ end of exon 2 were also detected in the top bands from RNAs of ALDH3-posittive tissues except the TCDD-treated Hep G2 cells.