FIG. 5.

Mapping of the transcription initiation sites for the human ALDH3 gene by primer extension analysis. The sizes of the oligomer primers and the extended bands corresponding to the 5′-RACE and other characterized cDNA clones are indicated at both sides. Total RNA (80 μg) was primed and reverse transcribed with 200 units of Superscriptll reverse transcriptase for 1 h at 42 °C. The primer extension products were analyzed on a 6% sequencing gel.