Fig. 7.
MIF-2/D-DT treatment stimulates proliferation in mouse proximal tubular (MPT) cells. A: immunofluoresence stain for the cell proliferation marker 5-bromo-2′-deoxyuridine (BrdU) in hypoxic MPT cells. Right: BrdU-positive cells at 24 and 36 h posthypoxia with MIF-2/D-DT treatment. Left: untreated controls. B: analysis of BrdU-positive cells with MIF-2/D-DT treatment compared with untreated controls. At 36 h posthypoxia, MIF-2/D-DT-treated cells showed a significant increase in proliferating cell numbers. BrdU-positive cell ratio calculated as described in methods. *P < 0.05; n = 3–4 samples. C: IF stain for Ki-67 in MPT cells 36 h posthypoxia. Right: Ki-67-positive cells after MIF-2/D-DT treatment (purple nuclei). D: analysis of Ki-67-positive cells with MIF-2/D-DT treatment compared with untreated controls. At 36 h posthypoxia, MIF-2/D-DT treated cells showed a significant increase in Ki-67-positive cells. *P < 0.05; n = 3–4 samples.