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. 2018 Apr 6;114(12):1642–1655. doi: 10.1093/cvr/cvy075

Figure 6.

Figure 6

miR-214 regulates PTEN level and of CMs proliferation. (A) Immunostaining for EdU in myocytes transfected with miR-214, inhibitor or control (bars, 40 μm). White arrows refer to EdU-positive myocytes. (B) Quantification of EdU-positive myocytes. *P < 0.05 vs. control; n = 6 per group (one-way ANOVA analysis with Bonferroni’s multiple comparison test). (C) qPCR for miR-214 in foetal and adult rats. *P < 0.05 vs. adult rat; n = 6 per group (Student’s t-test). (D) PTEN contains a site complementary to the seed sequence of miR-214. (E) Expression of target genes after overexpression of miR-214 according to qPCR. *P < 0.05 vs. control; n = 6 per group (Student’s t-test). (F) PTEN and cyclin D1 protein level when miR-214 was inhibited and overexpressed (western blot) (GAPDH internal reference). *P < 0.05 vs. mNC or iNC; n = 6 per group (Student’s t-test). (G) Location of miR-214 in CMs by qPCR. *P < 0.05 vs. nucleus; n = 6 per group (Student’s t-test).