Fig 5.

Decreased class-switched memory B-cell and increased CD21^low B-cell counts in NFKB1 LOF variant carriers. A, Absolute numbers of CD19⁺ B cells; each dot represents a single subject and his or her age. Age-dependent reference values are shown in gray. B-E, Percentages within CD19⁺CD20⁺ B lymphocytes of CD27⁺IgD⁺ (nonswitched memory or marginal-zone B cells) and CD27⁺IgD⁻ (switched memory B cells; Fig 5, B) or CD27⁺IgG⁺ (Fig 5, C), CD27⁺IgA⁺ (Fig 5, D), or CD21^lowCD38^low/dim (Fig 5, E). CA, Clinically affected subjects with an LOF variant in NFKB1; CU, clinically unaffected subjects with an LOF variant in NFKB1; HD, healthy donor. The gating strategy is shown in Fig E6, A. Only subjects with sufficient B cells could be analyzed. P values were determined by using 1-way (Fig 5, E) or 2-way (Fig 5, B) ANOVA with the Bonferroni post hoc test or unpaired Student t test (Fig 5, C and D). ns, Not significant. **P ≤ .01 and ***P ≤ .001.