Eye and lens phenotypes in reciprocal hybrids at 72-hours post-fertilization (hpf). A-D. Surface fish (SF) (A), surface fish x cavefish (SF X CF) hybrid (B), cavefish x surface fish (CF X SF) hybrid (C), and cavefish (CF) (D) larvae. Lens and retina are outlined by yellow dashes in A-D. Scale bar: 30 μm. E, F. Histograms comparing eye (E) and lens (F) sizes in SF, SF X CF hybrids, CF X SF hybrids, and CF. The number of larvae is shown at the base of each histogram. A total of 63 hybrids from 3 reciprocal hybridizations were analyzed. Error bars: standard errors of the means. * denotes significance at p < 0.05. *** denotes significance at p < 0.001. n.s.: no significance. See Table S3 for statistical scores.