Table 2.

Touchdown-PCR setup

Step	Temperature (°C)	Time	Note
1	94	3 min	-
2	94	20 s	-
3	64	30 s	-0.5 °C per cycle
4	72	35 s	Repeat steps 2-4 for 12 cycles
5	94	20 s	-
6	58	30 s	-
7	72	35 s	Repeat steps 5-7 for 25 cycles
8	72	2 min	-
9	4		Hold