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(A) Schematic overview of the pH effect on the reaction of tetrazine 2c with VBA 1 yielding dihydropyridazine 7. (B) Reactions of tetrazine 2c with VBA 1 (left) and norbornene 8 (right) in 50% MeOH and 50% Na2B4O7 buffer, ranging from pH 8 until 11. The reactions were measured at 20 °C by following the decay of the tetrazine at 540 nm. [a] Endo/exo 2:1.
