Fig. 5. (a) Chemical structure of complex 3a and the structural and spectral (in CH3OH) responses toward hypochlorite. (b) Images of living HeLa cells incubated with complex 3a (5 μM, 20 min, 37 °C) followed by treatment with NaClO (25 μM, 20 min, 37 °C) and costaining with CellMask (CM). (c) Images of living HeLa cells preincubated with NaClO (25 μM, 20 min, 37 °C), washed with PBS three times, incubated with complex 3a (5 μM, 20 min, 37 °C) and costained with CellMask. (d) Laser-scanning luminescence confocal microscopy images of living HeLa cells treated with elesclomol (125 nM, 2 h, 37 °C) followed by incubation with complex 3a (5 μM, 20 min, 37 °C) and costaining with CellMask. OL: overlaid images. Percentage values: co-localization coefficients. Scale bar: 20 μm.
