TABLE 1.
Strains, plasmids, and oligonucleotides
| Strain, plasmid, or oligonucleotide | Relevant characteristic(s)a | Source or reference |
|---|---|---|
| Strains | ||
| RN4220 | Highly transformable S. aureus | 52 |
| AH1263 | USA300 CA-MRSA strain LAC without LAC-p03 | 53 |
| JLB2 | AH1263 ΔfakA | 27 |
| JLB113 | AH1263 ccpA::tetL | 12 |
| JLB114 | AH1263 ΔfakA ccpA::tetL | This study |
| JLB127 | AH1263 ackA::ΝΣ | This study |
| JLB128 | AH1263 ΔfakA ackA::ΝΣ | This study |
| JLB132 | AH1263 ureB::ΝΣ | This study |
| JLB133 | AH1263 ΔfakA ureB::ΝΣ | This study |
| JLB158 | AH1263 gudB::ΝΣ | This study |
| JLB159 | AH1263 ΔfakA gudB::ΝΣ | This study |
| NE385 | Source of acsA::ΝΣ | 54 |
| NE1397 | Source of alsS::ΝΣ | 54 |
| NE1642 | Source of ureB::ΝΣ | 54 |
| KB8007 | JE2-ΔackA::ermB; Emr | 33 |
| JLB165 | AH1263 codY::ΝΣ | This study |
| JLB166 | AH1263 ΔfakA codY::ΝΣ | This study |
| NE1518 | Source of gudB::ΝΣ | 54 |
| NE1555 | Source of codY::ΝΣ | 54 |
| NE1670 | Source of ccpA::ΝΣ | 54 |
| NE1923 | Source of ldh1::ΝΣ | 54 |
| NE1816 | Source of ldh2::ΝΣ | 54 |
| JLB115 | AH1263 saeR::ΝΣ | 30 |
| JLB116 | AH1263 ΔfakA saeR::ΝΣ | 30 |
| JLB115 | AH1263 acsA::ΝΣ | This study |
| JLB116 | AH1263 ΔfakA acsA::ΝΣ | This study |
| JLB188 | AH1263 ldh1::ΝΣ | This study |
| JLB189 | AH1263 ΔfakA ldh1::ΝΣ | This study |
| JLB196 | AH1263 ldh2::ΝΣ | This study |
| JLB197 | AH1263 ΔfakA ldh2::ΝΣ | This study |
| JLB135 | AH1263 alsS::ΝΣ | This study |
| JLB136 | AH1263 ΔfakA alsS::ΝΣ | This study |
| Plasmids | ||
| pJB185 | Promoterless codon-optimized lacZ, Amp/Cmr | 30 |
| pJB165 | fakA complement, Amp/Cmr | 27 |
| pZD3 | Pure-lacZ reporter, Amp/Cmr | This study |
| Oligonucleotides | ||
| RT_ilvD_F | GGACCAGGTATGCCTGAAAT | This study |
| RT_ilvD_R | GGGGAAATATGACCAACTGC | This study |
| RT_gltA_F | TGGAAAAACGTATGGCAGAA | This study |
| RT_gltA_R | CCATCCTGCAGAACGACTTA | This study |
| RT-sigA-F | AACTGAATCCAAGTGATCTTAGTG | 55 |
| RT-sigA-R | TCATCACCTTGTTCAATACGTTTG | 55 |
| Purease-F2 | aatgaattcGATGATGCTACCTACAATAGCCGCTA | This study |
| Purease-R2 | ggacgtcgacCCAATTTCATATTAGATACAATTTACAAAATT | This study |
| CNK61 | GATATGCCATTTCCAACTGTCG | This study |
| CNK63 | GCTGCAAGTTGATCCATAGAGG | This study |
a
Antibiotic resistance abbreviations used: Amp/Cmr, ampicillin and chloramphenicol resistant; Emr, erythromycin resistant; tetL, tetracycline resistance gene. Oligonucleotide sequences are provided in the 5′-to-3′ orientation. Lowercase indicates nonhomologous sequences added for cloning purposes. CA-MRSA, community-associated methicillin-resistant Staphylococcus aureus.