Fig. 1. Fluorescent labeling and DNA replacement on the surface of zebrafish embryos. (a) Lipid DNA (U4T18) is anchored on the skin membrane of 1 dpf zebrafish embryos, and (b) hybridizes to 1 μM ATTO594 fluorescently labeled complementary DNA (C594) (step 1), resulting in red fluorescence on the zebrafish surface. (c) A 20-mer oligonucleotide replaces C594 by means of strand displacement (step 2), resulting in loss of fluorescence of the fish. (d) Addition of 1 μM ATTO488 fluorescently labeled complementary DNA (C488) results in hybridization with U4T18 (step 3) and the appearance of a green fluorescence at the zebrafish surface. The insert represents the sequences of C594, 20-mer and C488. Red channel = ATTO594; green channel = ATTO488.
