Table 2.
Meta-hemoglobin and oxy-hemoglobin formation in total hemoglobin content, supernatant (hemoglobin outside the cell) and pellet (hemoglobin inside the cell) after 4 hours of erythrocytes incubation (5% hematocrit) in the absence or presence of AAPH (50 mM).
| Experimental condition | Total hemoglobin content |
Supernatant |
Pellet |
|||
|---|---|---|---|---|---|---|
| MetHb (μM) |
OxyHb (μM) |
MetHb (μM) |
OxyHb (μM) |
MetHb (μM) |
OxyHb (μM) |
|
| Control | 19.6 ± 5.1∗∗ | 956.4 ± 40.6∗∗ | 3.3 ± 1.7∗∗ | 11.9 ± 3.2∗ | 28.8 ± 17.2 | 863.1 ± 36.7∗∗ |
| AAPH | 718.1 ± 66.6 | 171.1 ± 38.3 | 812.6 ± 94.7 | 189.1 ± 32.8 | 25.1 ± 4.6 | 7.4 ± 2.2 |
| AAPH +50 μg/mL OLE | 683.0 ± 70.1 | 250.3 ± 84.3 | 729.5 ± 47.5 | 281.4 ± 41.6∗ | 20.5 ± 7.5 | 7.5 ± 1.9 |
| AAPH +100 μg/mL OLE | 543.7 ± 117.1∗ | 447.6 ± 82.2∗∗ | 561.4 ± 78.7∗∗ | 371.7 ± 91.7∗∗ | 16.1 ± 5.5∗ | 13.2 ± 3.6 |
| AAPH +150 μg/mL OLE | 406.0 ± 93.2∗∗ | 612.4 ± 45.9∗∗ | 362.5 ± 17.6∗∗ | 387.8 ± 27.0∗∗ | 32.4 ± 18.8 | 172.9 ± 52.9∗ |
| AAPH +250 μg/mL OLE | 207.8 ± 33.5∗∗ | 712.0 ± 109.8∗∗ | 18.1 ± 6.1∗∗ | 4.1 ± 2.4∗ | 127.4 ± 31.4∗∗ | 635.9 ± 63.9∗∗ |
Values are expressed as mean ± standard deviation.
MetHb, meta-hemoglobin; OxyHb, oxy-hemoglobin; OLE, olive leaf extract.
Significantly different in relation to AAPH, in the same column: *p < 0.05; **p < 0.01 (t-test).