TABLE III.
Oligonucleotide Primers Used for PCR Amplification and/or for Sequencing of the 5-HT1B Receptor Gene*
| Primer designation | Type | Sequence | Length |
|---|---|---|---|
| H5HT1BR −595F | PCR and Sequencing | 5′-CAG CGC TGC TCC TAG ACT TC-3′ | 20 |
| H5HT1BR −174F | Sequencing | 5′-GGC TGC CGC ACC CAT GAC CT-3′ | 20 |
| H5HT1BR 178F | Sequencing | 5′-TCA CCG ACC TGC TTG TGT CC-3′ | 20 |
| H5HT1BR 864F | Sequencing | 5′-CCA AGT CAA AGT GCG AGT CT-3′ | 20 |
| H5HT1BR −40R | Sequencing | 5′-ATG GAG CGG ACG AAG GAG A-3′ | 19 |
| H5HT1BR 493R | Sequencing | 5′-TCT TGG GAG TCC TTT TAG C-3′ | 19 |
| H5HT1BR 784R | Sequencing | 5′-CGG GGG AGT CGG TTA TCA C-3′ | 19 |
| H5HT1BR 1247R | PCR and sequencing | 5′-TTC GAC CTA CCT GTG GAA CC-3′ | 20 |
*
The designation of primers corresponds to the position within the gene using the numbering scheme that designates the first nucleotide of the start codon as position +1 (F = forward; R = reverse).