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. 2018 Sep 20;11:521. doi: 10.1186/s13071-018-3092-1

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 11 — a Trypanosoma copemani infecting potoroo kidney epithelial (PtK2) cells in vitro after 24 h (fixed samples). Parasites and cells were stained with DAPI and Lysotracker®. DIC, DAPI, and Lysotracker® channels are split to show individual staining, and the merged panel is shown last. Arrows point to a cell with attached parasites and lysosomes that are not localised in the same place as parasite attachment. Cells are recognisable by their larger nuclei and parasites by their smaller nuclei stained with DAPI. b Trypanosoma cruzi infecting potoroo kidney epithelial (PtK2) cells in vitro after 72 h (fixed samples). Arrow indicates an infected cell. Parasites are recognisable by their smaller nucleus and kinetoplast stained with DAPI. Cells are distinguishable by their larger DAPI stained nuclei compared to the smaller parasite nuclei. Channels are split into DIC, DAPI and both channels merged. Scale-bar: 20 μm