Table 2.

Gradient program employed for the separation of PTC-amino acids

Time (min)	0	10	20	21	34	35	43	44	49	50	53
A% (v/v)	95	91	89	79	77	75	68	20	10	95	95
B% (v/v)	5	9	11	21	23	25	32	80	90	5	5