Skip to main content
. 2018 Jul 7;39(9):1185–1196. doi: 10.1093/carcin/bgy092

Figure 1.

Figure 1.

Immunoblotting for miRNA that regulate PTEN protein levels and miR-498 expression in BCa cell lines. (A) Summary of the screen for miRNA that regulate PTEN protein levels. (B) Endogenous PTEN protein levels were screened by immunoblotting after miRNA transfections in MCF-7 cells. (C) miR-498 expression profiles were determined by TaqMan probe qRT-PCR in the non-tumorigenic breast epithelial cell line MCF-10A and four TNBC cell lines as indicated. (D) Levels of endogenous PTEN protein expression were detected by western blotting in MCF-10A, MCF-7 and four TNBC cell lines. An antibody against β-actin was used as a loading control. (E) The levels of PTEN mRNA expression were detected by qRT-PCR in the indicated cell lines. (F and G) Kaplan–Meier survival curves generated from two datasets [TCGA (F) and GSE 40267 (G)] revealed that patients with BCa with high levels of miR-498 had a significantly lower overall survival (kmplot.com, 30 31). C and E are results obtained from three independent repeats.