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. 2018 Jul 7;39(9):1185–1196. doi: 10.1093/carcin/bgy092

Figure 4.

Figure 4.

miR-498 promotes cell proliferation and cell cycle arrest by suppressing PTEN and increasing p-Akt levels in TNBC cells. (A) MDA-MB-231 cells were transduced with the indicated expression plasmids and analyzed by immunoblotting with specific antibodies. (B) Levels of miR-498 expression were measured by qRT-PCR. (C) Similar to A, except that BT-549 (PTEN null) cells were used. (D) Levels of miR-498 expression were measured by qRT-PCR. (E) MDA-MB-231 cells were transduced with the indicated expression plasmids. Colony-forming assays were performed. Ratio of colony formation is shown in the right panel as indicated. (F) Similar to E, except that BT-549 cells were used. All experiments were performed in triplicate. *P < 0.05. **P < 0.01. (G) MDA-MB-231 cells were transduced with plasmids expressing miR-498 or control-miRNA. The cell cycle profile was determined by propidium iodide staining and flow cytometry (left panel). The cell cycle distribution was shown in the right panel. (H) Similar to G, except that BT-549 cells were used. **P < 0.001 (n = 3); the results are from three independent experiments.