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. 2018 Sep 20;18:145. doi: 10.1186/s12935-018-0604-1

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — miR-490-3p is essential for TGFβ1-induced EMT affected by CCAT1 knockdown via downregulation of TGFβR1. a Representative images of wound healing assay and quantification carried out in ovarian cancer cells (SKOV3 and CaOV3) transfected with sh-NC (scramble), sh-CCAT1 (CCAT1 knockdown), sh-CCAT1 (CCAT1 knockdown) plus miR-490-3p mimics, sh-CCAT1 (CCAT1 knockdown) plus miR-490-3p inhibitor. All cells were treated with 10 ng/ml TGFβ1. b Cell invasion assay and quantification showed invasiveness of ovarian cancer cells (SKOV3 and CaOV3) transfected with sh-NC (scramble), sh-CCAT1 (CCAT1 knockdown), sh-CCAT1 (CCAT1 knockdown) plus miR-490-3p mimics, sh-CCAT1 (CCAT1 knockdown) plus miR-490-3p inhibitor. All cells were treated with 10 ng/ml TGFβ1. c The mRNA level of TGFβR1 and EMT-associated markers (claudin, E-cadherin, N-cadherin, vimentin and MMP9) in ovarian cancer cells (SKOV3 and CaOV3) transfected with sh-NC (scramble), sh-CCAT1 (CCAT1 knockdown), sh-CCAT1 (CCAT1 knockdown) plus miR-490-3p mimics, sh-CCAT1 (CCAT1 knockdown) plus miR-490-3p inhibitor was detected by RT-qPCR. All cells were treated with 10 ng/ml TGFβ1. d Westernblot analysis showed the protein level of TGFβR1 and EMT-associated markers (claudin, E-cadherin, N-cadherin, vimentin and MMP9) in ovarian cancer cells (SKOV3 and CaOV3) transfected with sh-NC (scramble), sh-CCAT1 (CCAT1 knockdown), sh-CCAT1 (CCAT1 knockdown) plus miR-490-3p mimics, sh-CCAT1 (CCAT1 knockdown) plus miR-490-3p inhibitor. All cells were treated with 10 ng/ml TGFβ1. All data were represented as mean ± SD from three biological replicates (*P < 0.05; **P < 0.01)