Table 1.

Plasmids and strains used in this study

Plasmid or strain	Relevant feature
Plasmid
pIJ350	Versatile vector for protein expression; thiostrepton resistance marker
pUC19	Vector for cloning genes
pGM160	A shuttle vector containing pSG5 ori
pK18mob	Cloning vector; oriT, oriV; kanamycin resistance marker
pXC	Vector for expression of chitobiase gene (SAV_5268) under the control of xylA promoter; thiostrepton resistance marker
pXCBR	Vector for expression of chitobiase gene (SAV_5268) under the control of xylA promoter and xylR gene under the control of xylB promoter; thiostrepton resistance marker
pLR	Vector containing pSG5 ori for expression of xylR gene under the control of pld promoter; kanamycin resistance marker
pXCPR	Vector for expression of chitobiase gene (SAV_5268) under the control of xylA promoter and xylR gene under the control of pld promoter; thiostrepton resistance marker
Strain
Escherichia coli strain JM109	recA1 endA1 gyrA96 thi-1 hsdR17 (rK− mK+) e14− (mcrA−) supE44 relA1 Δ(lacproAB)/F’[traD36 proAB + lacI q lacZΔM15]
S. lividans 1326	WT strain
XC	Streptomyces lividans strain harboring pXC vector
XCBR	Streptomyces lividans strain harboring pXCBR vector
XCr	Streptomyces lividans strain harboring pXC and pLR vectors
XCPR	Streptomyces lividans strain harboring pXCPR vector