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Experimental details of permanent elution (PE).
| Number of protocol/column | Volume of eluant [mL]a) | Interval between elution steps [min] |
Total time of ten elution steps [min] |
Drying |
|---|---|---|---|---|
| S 1 | 1 | 5 | 60 | No special treatment |
| S 2 | 25 | 5 | 300 | No special treatment |
| S 3 | 1 | 30 | 310 | No special treatment |
| S 4 | 1 | 5 | 60 | Nitrogen stream |
| S 5 | 1 | 5 | 60 | Hot air stream |
a) Flow rate: 1 mL/min
