Fig. 6.

Insulin secretion 7 and 14 days after incubation. (A) Insulin secretion per INS-1 cell aggregate with 1.0 × 10¹ of middle size (□) and large size gelatin hydrogel microspheres (△) or without gelatin hydrogel microspheres (〇) at the initial seeding density of 1.0 × 10³ cells/well. *p < 0.05, †p < 0.01, significant against insulin secretion per aggregate without gelatin hydrogel microspheres at the corresponding time. ‡p < 0.05, significant against insulin secretion per aggregate with middle size of gelatin hydrogel microspheres at the corresponding time. (B) Insulin secretion per INS-1 cell aggregate with 1.0 × 10² of middle size (■), 0.5 × 10² of large size (▲) and 1.0 × 10² of large size of gelatin hydrogel microspheres (▽) or without gelatin hydrogel microspheres (●) at the initial seeding density of 1.0 × 10⁴ cells/well. (C) Insulin secretion per INS-1 cell in aggregates with 1.0 × 10¹ of middle size (□) and large size gelatin hydrogel microspheres (△) or without gelatin hydrogel microspheres (〇) at the initial seeding density of 1.0 × 10³ cells/well. §p < 0.05, significant against insulin secretion per aggregate without gelatin hydrogel microspheres at the corresponding time. ||p < 0.05, significant against insulin secretion per aggregate with middle size of gelatin hydrogel microspheres at the corresponding time. (D) Insulin secretion per INS-1 cell in aggregates with 1.0 × 10² of middle size (■), 0.5 × 10² of large size (▲) and 1.0 × 10² of large size of gelatin hydrogel microspheres (▽) or without gelatin hydrogel microspheres (●) at the initial seeding density of 1.0 × 10⁴ cells/well.