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Analysis of colony-forming cells in the anterior cruciate ligament (ACL). (a) Schema of cell isolation from the ACL. (b) Representative flow cytometric profiles of freshly isolated ACL-derived cells stained for CD29, CD44, CD73, CD90, CD105, CD106, CD140a, CD146, CD166, and CD271 (grey: isotype control; red: sample). (c) Colony formation rates during 3 weeks of culture after cell sorting.
