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Purification of ACL-derived mesenchymal stem/stromal cells (MSCs) using surface markers. (a, b) Representative flow cytometric profiles of fresh ACL-derived cells stained for CD29 and CD90 and gated for the CD73+ (a) and CD73+CD90+ fractions (b). (c) Rate of colony formation on day 21 in the following cells: CD73+/90+, +/−, −/+, −/−, and propidium iodide (PI)− (n = 3–7, p* < 0.05, p*** < 0.001, Student's t-test with Bonferroni's correction).
