Fig. 5.
Role of pyruvate decarboxylase (PDC6) on pigmentation and growth on 102a (spt15_Ala101Thr) mutant strain. a Effect of overexpression of spt15_Ala101Thr plasmid on the growth of BY4741 (wild type) and pdc1Δ, pdc5Δ and pdc6Δ deletion strains (containing carotenogenic genes (TPSY1 and GPDCRTI_Ala393Thr)) b Effect of overexpression of PDC6 alone and in combination with either SPT15WT or spt15_Ala101Thr plasmid on pigmentation of carotenogenic yeast. c Amount of carotenoids in the carotenogenic yeast strains overexpressing either spt15_Ala101Thr alone or in combination with PDC6. In a, red box depicts the growth defect observed in spt15_Ala101Thr mutant strain in pdc6Δ deletion strain under low phosphate conditions and blue box depicts slight growth defect observed in SPT15WT strain in pdc5Δ deletion strain under low phosphate conditions. In b, green boxes depict the two spt15_Ala101Thr mutant strains with and without overexpression of PDC6, T refers to TEF promoter