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. Author manuscript; available in PMC: 2019 Aug 2.
Published in final edited form as: Cell Stem Cell. 2018 Jun 28;23(2):252–265.e8. doi: 10.1016/j.stem.2018.06.001

Figure 4. Id1 expression is upregulated in hematopoietic stem and progenitor cells by cytokines and proinflammatory agents.

Figure 4.

(A) Expression of Id1(GFP) in HSPCs in Id1GFP/+ mice (n=5 mice/group). (B) Cytokine/growth factor induced Id1(GFP) expression in HSCs from Lin- Id1GFP/+ reporter BMC cultures. Dashed line indicates percent Id1(GFP) expression for cells cultured in SCF alone. (C) Cytokine/growth factor induced proliferation of Id1(GFP+) and Id1(GFP−) HSCs from Lin- Id1GFP/+ reporter BMC cultures. (D) γ-irradiation, cytokines and proinflammatory mediator induction of Id1(GFP) expression in HSCs from Id1GFP/+ reporter BMCs in vivo. Dashed lined indicates control value for mice treated with saline (n=5 mice/group). (E) Effect of pan-JAK and pan-STAT inhibitors on the induction of Id1(GFP) expression in HSCs following BMT of Id1GFP/+ reporter BMCs. (n=5 mice/group). See also Figure S6.