Figure 5. Increased survival and quiescence of Id1−/− HSCs in vitro.
(A) Total number of HSCs present in Id1+/+ and Id1−/− LSK cell cultures after 6 days in vitro culture. (B) HSC cycling in LSK cell cultures after 6 days determined by Ki-67/FxCycle staining, and (C) proliferation by BrdU incorporation. (D) HSC single cell division assay. (E) Total number of HSCs in Id1−/− and Id1−/− chimeric mice treated with LPS (0.043 mg/kg) every other day for 30 days (n=5 mice/group). Dashed lines indicate values from untreated control mice. See Figure S6C.