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. 2017 Nov 13;12(3):236–246. doi: 10.1080/19336918.2017.1365992

Figure 3.

Figure 3.

The TGFBR3 gene is a direct target for miR-19a and miR-424. (A) Sequence analysis revealed the two binding sites of miRNA: mRNA complementary between miR-19a, miR-424, and human TGFBR3 gene. (B) Compared with the control, transfection of miR-19a or miR-424 resulted in a significant reduction in TGFBR3 protein expression. Co-transfection of miR-19a with AMO-19a or miR-424 with AMO-424 alleviated the reduction in TGFBR3 protein expression. (C) qRT-PCR analysis revealed that miR-19a and miR-424 markedly inhibited TGFBR3 mRNA expression. (D) Compared with the control, transfection of miR-19a or miR-424 with the luciferase reporter gene linked to the wild-type 3′-UTR of TGFBR3 resulted in a significant reduction in luciferase activity. Co-transfection of miR-19a with AMO-19a or miR-424 with AMO-424 alleviated the reduction in luciferase activity. Three independent experiments (n = 3) were performed for each condition. Data are shown as the mean ± SEM. *p < 0.05 vs. control,# p < 0.05 vs. miR-19a or miR-424 correspondingly. Ctl control, NC transfection of negative control, miR-19a transfection of miR-19a, +AMO-19a co-transfection of miR-19a and its inhibitor (AMO-19a), miR-424 transfection of miR-424, +AMO-424 co-transfection of miR-424 and its inhibitor (AMO-424).