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. Author manuscript; available in PMC: 2019 Mar 7.
Published in final edited form as: Science. 2018 Sep 7;361(6406):eaan8821. doi: 10.1126/science.aan8821

Fig. 5. Ablating AHN in male 5×FAD mice reduces hippocampal levels of TGF-β1, a protective cytokine.

Fig. 5.

(A) Hippocampal TGF-β1 levels in male 5×FADSham and 5×FADIR (left), 5×FADVeh and 5×FADTMZ (High KD) (middle), and 5×FADLV-GFP and 5×FADLV-dnWnt (High KD) mice (right). Levels as % of 5×FAD control group in each treatment. (B) Photomicrographs of 3D6+ Aβ plaques and NeuN+ cells (left), DCX+ cells (middle), and Casp3+ cell and NeuN+ cells (right) in the transduced DG of 5×FADIR mice by LV-TGF-β1. Scale bars: 50 μm (left, middle); 20 μm (right). (C) Hippocampal TGF-β1 levels in 5×FADIR/LV-RFP (n = 8) and 5×FADIR/LV-TGF-β1 (n = 8) (left), 5×FADTMZ/LV-RFP (n = 9) and 5×FADTMZ/LV-TGF-β1 (n = 12) (middle), and 5×FADLV-dnWnt/LV-RFP (n = 8) and 5×FADLV-dnWnt/LV-TGF-β1 (n = 9) (right) mice. Levels as % of 5×FAD control group in each treatment. 5×FADTMZ/LV-RFP and 5×FADLV-dnWnt/LV-RFP mice with moderate AHN knockdown were not included. (D) Quantification of Casp3+ cells in 5×FADIR/LV-RFP and 5×FADIR/LV-TGF-β1 (left), 5×FADTMZ/LV-RFP and 5×FADTMZ/LV-TGF-β1 (middle), and 5×FADLV-dnWnt/LV-RFP and 5×FADLV-dnWnt/LV-TGF-β1 (right) mice. (E) Representative images of Casp3+ cells (arrows) in GFP-labeled 3D-FAD cell cultures. Scale bar: 50 μm. (F) Quantification of Casp3+ cells in 3D-FAD cell cultures treated with vehicle (veh) or TGF-β1 (10 ng/ml). n = 3 per group. (G) Representative images of DAPI+ cells (blue) in ReN-FAD cell cultures treated with vehicle or TGF-β1. Scale bar: 50 μm. (H) Number of cells survived in 3D-FAD cultures treated with vehicle or TGF-β1. n = 3 per group.