Figure 9.

Classification criteria of GV oocytes. (A) COCs control, with an unexpanded cumulus mass having four or more layers of cumulus cells surrounding the zona pellucida and with homogenous cytoplasm; (B) COCs control with an unexpanded cumulus mass having three to four layers of cumulus cells and with relative homogenous cytoplasm; (C) oocytes with an unexpanded cumulus mass having under three layers of cumulus cells with regular cytoplasm or irregular shrunken cytoplasm; (D) oocytes partially denuded of cumulus cells and with irregular shrunken cytoplasm; and (E) oocytes completely denuded of cumulus cells and with irregular shrunken cytoplasm. C,D and E were classified as IOs. scale bar = 100 μm.